Fig. 6: Experimental analysis with DBOpt.

a dSTORM image (Integrin 02, Table S3) of a cell co-labeled for vinculin (red) and active β1 integrin (cyan). (scale bar: 10 μm) b Selected ROI (white box in (a); scale bar: 2 μm). c DBOpt parameter sweep of Integrin 02 localizations with DBSCAN. d Selected region (corresponding to (b)) of identified integrin clusters from DBOpt; color depicts individual cluster score. e Distribution of individual integrin cluster scores from vinculin ROIs; clusters scoring ≥ 0 were analyzed for size (n = 3462 clusters not excluded, pooled from five samples). f Width, length, and aspect ratio (AR) of all integrin clusters from vinculin ROIs (n = 3462 clusters, pooled from five samples). Box plot lines indicate median, “X” indicates mean, boxes represent 25th and 75th percentiles, and whiskers represent 5th and 95th percentiles. g dSTORM image (Clathrin 01, Table S3) of a cell labeled for clathrin (red) (scale bar: 10 μm). h Selected ROI (white box in (g); scale bar: 2 μm)) with inset showing a 100 nm axial region of a clathrin structure projected in 2D (scale bar: 0.2 μm). i DBOpt parameter sweep of Clathrin 01 localizations with DBSCAN. j Selected region of identified clathrin clusters from DBOpt; color depicts individual cluster score. k Distribution of individual cluster scores from full cell results; clusters scoring < 0 were excluded (n = 2045 clusters not excluded, pooled from five samples). l FWHMs of clathrin clusters from full cell results (n = 2045 clusters not excluded, pooled from five samples); inset depicts results with individual cluster scores < 0.5 excluded (n = 1406 clusters not excluded, pooled from five samples).