Fig. 1: CCDC91 is upregulated in HCC tissues and is correlated with a poor prognosis. | Communications Biology

Fig. 1: CCDC91 is upregulated in HCC tissues and is correlated with a poor prognosis.

From: HBV DNA integration gene CCDC91 is oncogenic and a potential therapeutic target for hepatocellular carcinoma

Fig. 1

A Map of the CCDC91 gene with HBV DNA insertion in HCC (n = 17) and NHL (n = 1) tissues. Black arrows indicate HBV integration sites identified through pooled-analysis in HCC. Red arrows represent HBV integration sites detected in HCC samples using our HBV capture sequencing approach. Blue arrows denote HBV integration sites identified in non-Hodgkin lymphoma (NHL) samples from our previous HBV capture sequencing study. B Immunohistochemistry staining showed the protein expression of CCDC91 in 4 HBV capture sequencing HCC tissues. C The mRNA levels of CCDC91 in HCC tissues and non-cancerous liver tissues from GSEA cohorts (GSE36376: n = 193 in non-tumor, n = 240 in tumor; GSE76427: n = 52 in non-tumor, n = 115 in tumor). D The mRNA levels of CCDC91 in HCC tissues and non-cancerous liver tissues from TCGA cohort (n = 50 in non-tumor, n = 371 in tumor). E Relative mRNA levels of CCDC91 in HCC cells as determined by qPCR (n = 3 independent biological repeats). F Representative IHC images of CCDC91 in HCC tissues and adjacent paratumor tissues (left) and IHC scores of CCDC91 staining in HCC tissues and non-cancerous liver tissues (right, n = 123). G The Kaplan–Meier analysis and log-rank test revealed the association of CCDC91 with the overall survival of HCC patients from TCGA dataset. H Multivariate logistic regression analyses for the associations of CCDC91 expression in HCC patients from TCGA dataset (n = 240). I Multivariate Cox regression analysis of the data from the TCGA dataset revealed that CCDC91 was an independent predictor of OS (n = 120). J The Kaplan–Meier analysis and log-rank test revealed the association of CCDC91 with the overall survival of HCC patients from TMA cohort (n = 123). K Multivariate logistic regression analyses for the associations of CCDC91 expression in HCC patients from TMA cohort (n = 80). Statistical analysis was performed using Student’s t test in (C, D, E, and F). Error bars represent the SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

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