Fig. 3: CCDC91 promotes cell proliferation in vivo.

AāC The subcutaneous tumor model formed by Huh-7-shCCDC91 and control Huh-7-SC cells in BALB/c nude mice (nā=ā5 per group). A The tumor growth curves based on tumor sizes measured at a 5-day interval. B Representative images of tumors at the time of sacrifice 36 days post tumor cells inoculation. C The tumor weights at the time of sacrifice. D, E The subcutaneous tumor model formed by HepG2-CCDC91 and control HepG2-VC cells in BALB/c nude mice. Representative images were shown (D) and the tumors with xenografts were weighted (E) (nā=ā5, respectively). F Representative images of IHC staining of CCDC91 and Ki-67 in the HCC tumors. G Quantification of the percentage of Ki-67-positive cells and CCDC91-positive cells. Statistical analysis was performed using Studentās t test in (C, E, and G). Two-way ANOVA was used for tumor volume in (A). Data represents meanā±āSEM.*, pā<ā0.05; **, pā<ā0.01; ***, pā<ā0.001.