Fig. 7: Ct-HBx mediates HBV integrated CCDC91 overexpression in HCC cells. | Communications Biology

Fig. 7: Ct-HBx mediates HBV integrated CCDC91 overexpression in HCC cells.

From: HBV DNA integration gene CCDC91 is oncogenic and a potential therapeutic target for hepatocellular carcinoma

Fig. 7

A The protein level of HBx in HepG2 and PLC/PRF/5 cells with or without Ct-HBx transfection detected by western blot (n = 3). B The mRNA level of CCDC91 in HepG2 cells with or without Ct-HBx transfection revealed by qPCR (n = 3). C Co-IP with antibody against HBx followed by Western blot in HepG2 and PLC/PRF/5 cells (n = 3). D Immunofluorescence staining of CCDC91 and HBx in HepG2 and PLC/PRF/5 cells with or without Ct-HBx transfection. E Cell proliferation of HepG2 and PLC/PRF/5 cells with or without Ct-HBx transfection detected by CCK8 assay (n = 3). F, G) The migration and invasion abilities of HepG2 and PLC/PRF/5 cells with or without Ct-HBx transfection measured by transwell assay (n = 3). H The protein level of HBx, CCDC91, and LDHA in HepG2 and PLC/PRF/5 cells with or without Ct-HBx transfection revealed by western blot (n = 3). I Schematic diagram illustrating the proposed mechanisms of the effects of the HBV-integrated gene CCDC91 on HCC. Statistical analysis was performed using Student’s t test in (B, E, F, and G). Data represents mean ± SEM.*, p < 0.05; **, p < 0.01; ***, p < 0.001.

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