Fig. 2: Characterization of TS^CV cells.

A Stem state phase contrast images of four chorionic villus-derived TS cell lines (TS^CVK01, TS^CVK09, TS^CVK23, TS^CVK24) alongside images of the reference cytotrophoblast-derived TS cell lines (TS^CT27 and TS^CT29) at different passage numbers (15–21). Scale bars represent 250 μm. B Bar graphs depicting expression of three microRNAs (miR) from the C19MC cluster (hsa-miR-517a-3p, has-miR-517-5p, and hsa-miR-526b-3p) in TS^CT and TS^CV cell lines relative to induced pluripotent stem (iPS) cells, measured by RT-qPCR. Data were normalized to the control miRNA, hsa-miR-103a-3p. Data are presented as the mean ± SEM. Each dot represents a biological replicate, n = 3 samples per group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. C Plots representing DNA methylation levels in the ELF5 promoter at 11 sites in TS^CT and TS^CV cell lines compared to iPS cells. Methylated sites (black) and unmethylated sites (white) are shown for 10 replicates, and the average percent methylation is listed.