Fig. 2: Quantitative proteomic profiling of Eml1 cKO neural progenitor cells.
From: Loss of Eml1 alters microtubule-associated protein networks in mouse brain heterotopia
A Overview of proteomic workflow for mouse neural progenitor cells derived from control and Eml1 cKO embryos. Pax6+ neural progenitor cells were isolated at E14.5 and propagated in culture. Cells were obtained from 6 WT and 6 Eml1 cKO embryos collected from 3 different litters. Each replicate was generated by pooling 3 embryos per genotype. Proteins were extracted, labeled using dimethyl isotopic labeling and fractionated by offline high-pH reverse phase prior to LC-MS/MS analysis. Created in BioRender. Ozlu, N. (2025) https://BioRender.com/5x8vohl. B Identification of 266 significantly dysregulated proteins out of nearly 4000 quantified proteins in Eml1 cKO neural progenitor cells. C Functional network analysis of the 266 dysregulated proteins generated with ClueGO in Cytoscape, highlighting enriched functional categories. D PANTHER GO-Slim Gene ontology (GO) enrichment analysis for biological process (BP), cellular component (CC), and molecular function (MF) for the upregulated (135 proteins) (brown) and downregulated (131 proteins) (blue) proteins in Eml1 cKO neural progenitor cells, respectively.
