Fig. 5: Analysis of differentially expressed genes (DEGs) in IGF2-overexpression and deficiency mice.

A, E A heat map of log(TPM) expression values of 50 statistically significant differentially expressed genes in the eWAT of mice overexpressing IGF2 and IGF2 knocking down mice with their control mice (T-test q-value < 0.05 adjusted with FDR multiple hypothesis correction), n = 2 with 2 replicate sequencing. B, C GO and KEGG assay of DEGs in the eWAT of mice overexpressing IGF2 and control mice. D The validated differential expression levels of genes associated with adipogenesis, lipogenesis, and lipolysis in the eWAT of mice overexpressing IGF2 and control mice, using Ppia as internal controls, n = 6. *p < 0.05, **p < 0.01 by two-tailed, unpaired Student’s t test. F, G GO and KEGG assay of DEGs in the eWAT of mice subjected to IGF2-RNAi and control mice. H The validated differential expression levels of genes associated with adipogenesis, lipogenesis, and lipolysis in the eWAT of mice subjected to IGF2-RNAi and control mice, using Ppia as internal controls respectively, n = 6. Data represent the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 by two-tailed, unpaired Student’s t test.