Fig. 4: Genotyping of mCherry-Tg mice with or without mutation.
From: Abnormal development of gastrointestinal system of homozygous Foxp2(R552H)-mutated mice
Foxp2R552H/+ mice were mated with mCherry-Tg mice, and Foxp2 R552H/+/mCherry-Tg mice, were further mated with Foxp2R552H/+ mice and their pups were genotyped by PCR. Images show detection of Foxp2, Foxp2(R552H), and mCherry in the pups. Upper panel: Arrow indicates mCherry. Tg, mCherry-Tg mice; Wt, mice expressing no mCherry. Lower panel: Closed arrowhead indicates bands from Wt. Open arrowhead indicates bands from Foxp2R552H/R552H. Wt, mice expressing wild-type Foxp2 (Foxp2+/+); He, heterozygous mice for Foxp2R552H/+; Ho, homozygous mice for Foxp2R552H/R552H. M, 100 bp DNA ladder marker.
