Fig. 7: The influence of Foxp2[R552H] mutation on the stomach epithelium.
From: Abnormal development of gastrointestinal system of homozygous Foxp2(R552H)-mutated mice
a Left panel; an illustration indicating the position of the sections used for immunostaining (marked with a blue dotted line). Right panel; immunostaining of the longitudinal section of the stomach in Foxp2+/+/mCherry-Tg (Wt) (i, iii, v) and Foxp2R552H/R552H/mCherry-Tg (Ho) mice (ii, iv, vi) at postnatal day (P) 28. Foxp2R552H/R552H (Ho) mice showed reduced expressions of SMA, E-cadherin, ZO-1 (green), and mCherry (red). Bars indicate 50 µm. b qPCR analysis reveals gene expressions in the stomach of Foxp2+/+ (Wt) and Foxp2R552H/R552H (Ho) mice (N = 5 per group). Each performed three times. Data represent mean ± standard error (SEM). For mCherry: Wt: 1.000 ± 0.086, Ho: 0.366 ± 0.033. For SMA: Wt: 1.000 ± 0.064, Ho: 0.566 ± 0.018. For E-cadherin: Wt: 1.000 ± 0.053, Ho: 0.362 ± 0.058. For Zo-1: Wt: 1.000 ± 0.068, Ho: 0.575 ± 0.043. Mann–Whitney U test; **P < 0.01. c The Zonulin measurement. The amount of Zonulin was higher in the Foxp2R552H/R552H (Ho) mice than in the Foxp2+/+ (Wt) mice at P28. 7 mice (N = 7 per group). Each performed in triplicates. Mann-Whitney U test; *** P < 0.001. Wt: median=0.556, range:0.422–0.785, Ho: median=2.249, range: 1.672–3.239. Box-plot elements: center line, median; box limits, upper and lower quartiles; whiskers, 1.5x interquartile range; points, outliers.
