Fig. 5: Pathway map of C and P metabolism gene expression in R. irregularis.

Transcriptional responses of genes in R. irregularis a P transport and regulation and arginine biosynthesis and b fatty acid elongation and degradation, energy metabolism, TCA cycle, gluconeogenesis and glycolysis pathways, under low (35 μM KH₂PO₄) and high (700 μM KH₂PO₄) P availabilities. The heat map shows the log₂ of transcripts per kilobase million (TPM) calculated based on the initial reads count. The dashed lines represent simplified pathways. The asterisks indicate a significant difference in gene expression level between (2) P^Low RI⁺ RA⁺ and (1) P^Low RI⁺ RA⁻ treatments; between (4) P^High RI⁺ RA⁺ and (3) P^High RI⁺ RA⁻ treatments; and between (2) P^Low RI⁺ RA⁺ and (4) P^High RI⁺ RA⁺ treatments (DESeq2 test, *p < 0.05; **p < 0.01; ***p < 0.001, n = 4 biologically independent sample). Abbreviations: RI⁻ RA⁺ only R. aquatilis cells present in the HC, RI⁺ RA⁻ only R. irregularis hyphae present in the HC, RI⁺ RA⁺ both R. irregularis hyphae and R. aquatilis cells present in the HC.