Table 1 Strains used in this study

From: Tom40 functions as a channel for protein retrotranslocation in the mitochondria-associated degradation (MAD) pathway

Strain

Genotype

Source

BY4741

MATa his3∆1 leu2∆0 met15∆0 ura3∆0

Open Biosystems (Huntsville, AL)

PLY054

MATa his3∆1 leu2∆0 met15∆0 ura3∆0 doa1∆::LEU2 ho::TEF-UBI4-kanMX4

(Liao et al., 2020)

PLY119

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] doa1∆::kanMX6

(Liao et al., 2020)

PLY132

MATa his3∆1 leu2∆0 met15∆0 ura3∆0 TOM70-6xHis-kanMX6 KGD1-13xMYC-URA3

(Liao et al., 2020)

PLY134

MATa his3∆1 leu2∆0 met15∆0 ura3∆0 doa1∆::LEU2 ho::TEF-UBI4-kanMX4 TOM70-6xHIS-HIS3MX6 KGD1-13xMYC URA3

(Liao et al., 2020)

PLY144

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] KGD1-GFP-bleMX6

(Liao et al., 2020)

PLY145

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] doa1∆::kanMX6 KGD1-GFP::bleMX6

(Liao et al., 2020)

PLY149

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] PIM1-GFP-bleMX6

(Liao et al., 2020)

PLY150

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] doa1∆::kanMX6 PIM1-GFP::bleMX6

(Liao et al., 2020)

PLY187

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] KGD1-GFP-bleMX6 tom40 130/138 C

This study

PLY189

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] doa1∆::kanMX6 KGD1-GFP::bleMX6 tom40 130/138 C

This study

PLY193

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] PIM1-GFP-bleMX6 tom40 130/138 C

This study

PLY195

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] doa1∆::kanMX6 PIM1-GFP::bleMX6 tom40 130/138 C

This study

CTY210

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] doa1∆::kanMX6 KGD1-GFP::bleMX6 tom5∆::HphMX6

This study

CTY217

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] PIM1-GFP-bleMX6 tom5∆::HphMX6

This study

CTY220

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] doa1∆::kanMX6 PIM1-GFP::bleMX6 tom5∆::HphMX6

This study

CTY221

MATa lys2-810 leu2-3,-112 ura3-52 his3∆200 trp1-1[am] ubi1-∆1::TRP1 ubi2-∆2::ura3 ubi3-∆ub-2 ubi4-∆2::LEU2 + pUB221 [YEp pCUP16xHis-Myc-Ub URA3], pUB100 [YEp pGPDUBI1-tail HIS3] KGD1-GFP-bleMX6 tom5∆::HphMX6

This study

CTY234

MATa his3∆1 leu2∆0 met15∆0 ura3∆0 tom5∆::HphMX6

This study

CTY235

MATa his3∆1 leu2∆0 met15∆0 ura3∆0 doa1∆::LEU2 ho::TEF-UBI4-kanMX4 tom5∆::HphMX6

This study

CTY237

MATa his3∆1 leu2∆0 met15∆0 ura3∆0 TOM70-6xHis-kanMX6 KGD1-13xMYC-URA3 tom5∆::HphMX6

This study

CTY239

MATa his3∆1 leu2∆0 met15∆0 ura3∆0 doa1∆::LEU2 ho::TEF-UBI4-kanMX4 TOM70-6xHIS-HIS3MX6 KGD1-13xMYC URA3 tom5∆::HphMX6

This study

CTY250

MATa his3∆1 leu2∆0 met15∆0 ura3∆0 doa1∆::LEU2 ho::TEF-UBI4-kanMX4 KGD1-6xGly-6xHis-His3Mx6

This study

CTY251

MATa his3∆1 leu2∆0 met15∆0 ura3∆0 KGD1-6xGly-6xHis-His3Mx6

This study

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