Fig. 7: In vivo imaging and aortic histological analysis results. | Communications Biology

Fig. 7: In vivo imaging and aortic histological analysis results.

From: Targeting VCAM-1 with chimeric antigen receptor and regulatory T cell for abdominal aortic aneurysm treatment

Fig. 7: In vivo imaging and aortic histological analysis results.

Imaging results on days 1, 5, 9, and 14 after cell injection for groups A, A + T, and A + C (A); Imaging of major organs (liver, heart, lung, kidney, aorta, spleen) on day 14 after cell injection, placed in 6 cm culture dish showing positive signal distribution (B–I); Corresponding organs numbered as follows: 1-liver, 2-heart, 3-lung, 4-kidney, 5-aorta, 6-spleen (B-II); Imaging of the aortas of the three groups on day 14 after cell injection in 6 cm culture dish showing positive signal distribution (C); Representative images of aorta from each group (D); Statistical graph of maximum aortic outer diameter, following CAR-Treg cell therapy (E), the diameter of mouse AAA decreased significantly (Sham, N = 6; AAA, N = 6; AAA + Treg, N = 6; AAA + CAR-Treg, N = 6); The HE and EvG staining images of mouse aortas from each group (F, 100× and 400× magnification); Semi-quantitative analysis of elastic fiber rupture in the arterial wall (G); Immunohistochemical staining of arterial tissues for four different intervention or treatment strategies, IL-17a (H), CD3 (J), F4/80 (L) and ELA2 (N) expression was observed 100× and 400× magnification, and scoring results of immunohistochemical staining for IL-17a (I), CD3 (K), F4/80 (M) and ELA2 (O). *P < 0.05, **P < 0.01, ***P < 0.001.

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