Fig. 2: Analysis of RNA-seq and MeRIP-seq data from germ-free Drosophila.
a Principal component analysis (PCA) of RNA sequencing data in control and folate-fed group (n = 3). b Volcano plot of RNA sequencing data in control and folate-fed group. FDR < 0.05 and log2FC ≥ 0.5 were set as the significance thresholds. c KEGG analysis of RNA sequencing data showing functional pathways of different genes in control and folate-fed group. d RT-qPCR experiments to verify carbon metabolic pathway genes from control group and folate-fed group in S2 cells (n = 3). e Normalized frequency of m6A peaks across different regions of mRNA in GF-Control and GF-Folate group, respectively. f Representative consensus motifs and corresponding P-values of m6A peaks identified in control and folate-fed group. g Genomic annotations of m6A sites in GF-Control and GF-Folate group, respectively. h Venn diagram showing common and unique differential m6A peak in GF-Control and GF-Folate group, respectively. i GO enrichment analysis showing functional pathways of different m6A peak-containing genes in GF-Folate and GF-Control group, respectively. j Dot blot analysis of mRNA m6A methylation levels in Control-brain group and Folate-brain group, respectively (n = 3). Results were normalized to methylene blue staining. k RT-qPCR analysis of Dhfr mRNA expression levels in Control-brain group and Folate-brain group (n = 3). l RT-qPCR analysis of Mettl3 mRNA expression levels in Control-brain group and Folate-brain group (n = 3). m Western blot analysis of METTL3 in Control-brain group and Folate-brain group, respectively (n = 3). n RT-qPCR analysis to verify carbon metabolic pathway genes in Control-brain group and Folate-brain group (n = 3). Mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001, measured with the unpaired, two-tailed Student’s t-test.
