Fig. 4: Effect of folate-producing L. plantarum on neural mRNA m6A methylation levels in Drosophila.
a Experimental design of L. plantarum colonization in Drosophila. Adult Drosophila samples (the whole fly) with (WT-LP) or without (WT-Control) L. plantarum colonization were collected for Western assay/Dot blot assay and MeRIP-seq sequencing. The image is created by BioRender. b Dot blot of mRNA m6A methylation levels in WT-Control group and WT-LP group (n = 3). Results were normalized to SYBRTM Gold staining. Mean ± SEM; * P < 0.05, measured with the unpaired, two-tailed Student’s t-test. c Western blot analysis of METTL3 in WT-LP and WT-Control, respectively (n = 3). Mean ± SEM; * P < 0.05, measured with the unpaired, two-tailed Student’s t-test. d Venn diagram showing common and unique differential m6A peaks in WT-Control and WT-LP group, respectively. e GO enrichment analysis showing functional pathways of different m6A peak-containing genes.
