Fig. 4: Impact of clpX or clpC1 gene suppression on the susceptibility of Mab and Msm to ILE or ILF. | Communications Biology

Fig. 4: Impact of clpX or clpC1 gene suppression on the susceptibility of Mab and Msm to ILE or ILF.

From: Mutations in ClpC1 or ClpX subunit of caseinolytic protease confer resistance to ilamycins in mycobacteria

Fig. 4

a In vitro susceptibility of Mab to ILE or ILF upon aTc-induced gene suppression. b In vitro susceptibility of Msm to ILE or ILF upon aTc-induced gene suppression. The concentrations of ILE and ILF are in μg mL−1. *, Mab strains contained varying plasmids: vector, a vector control with the empty plasmid pLJR962; clpC1, pLJR962-clpC1 for inducible inhibition of the clpC1 gene; clpX, pLJR962-clpX for inducible inhibition of the clpX gene. #, Similarly, Msm strains were differentiated by the plasmids they harbored as that in Mab. c Intracellular susceptibility of Mab to ILE upon gene suppression in RAW264.7 macrophages. Survival rates were calculated as (CFU of ILE-treated group/CFU of untreated group) × 100%. Numbers following the drug names indicate the working concentrations (µg mL−1). Statistical significance was determined by two-way ANOVA followed by Tukey’s multiple comparisons test. Data are presented as mean ± SD from three technical replicates (n = 3). **P < 0.01; ****P < 0.0001.

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