Table 2 Summary of mutations of ClpC1 and ClpX in ILE- and ILF-resistant mutants

From: Mutations in ClpC1 or ClpX subunit of caseinolytic protease confer resistance to ilamycins in mycobacteria

Strains No.a

MICs (µg mL-1)

Mutation

ILE

ILF

ClpC1

clpC1

ClpX

clpX

MtbR1b

0.625

-

-

-

P30H

CCC→CAC

MtbR2b

20

-

F80I

TTT→ATT

P30H

CCC→CAC

MtbR3b

20

-

F80V

TTT→GTT

P30H

CCC→CAC

MtbR4b

0.1

-

F80I

TTT→ATT

-

-

MtbR5b

0.1

-

F80V

TTT→GTT

-

-

MtbR6b

0.1

-

F80L

TTT→CTT

-

-

MtbR7b

20

-

F80C

TTT→TGT

-

-

MabR1b

> 128

> 128

-

-

P30H

CCC→CAC

MabR2b

4

> 128

F2L

TTC→TCC

-

-

MabR3b

4

> 128

M1-FERF-F2

Ins: TTCGAGAGATTC

-

-

MabR4b

4

> 128

Q17R

CAA→CGA

-

-

MabR5b

> 128

> 128

H77D

CAC→GAC

-

-

MabR6b

> 128

> 128

L88R

CTG→CGG

-

-

MmrR1b

1

4

F2L

TTC→TCC

-

-

MmrR2b

10

40

H77D

CAC→GAC

-

-

MmrR3b

10

40

L88R

CTC→CGC

-

-

MsmR1c

0.5

20

F2S

TTT→TCT

-

-

MsmR2c

0.25

10

F2C

TTT→TGT

-

-

MsmR3c

1

20

M1-L-F2

Ins: GTT

-

-

MsmR4c

1

10

F5I

TTT→ATT

-

-

MsmR5c

0.5

40

F80S

TTC→TCC

-

-

MsmR6c

0.25

10

L88P

CTC→CCC

-

-

MsmR7c

0.25

20

L88R

CTC→CGC

-

-

MsmR8c

0.25

10

F2S; I197N

TTT→TCT;

ATC→AAC

-

-

  1. Underlines indicate mutated bases.
  2. Ins insertion, − showed no mutation.
  3. aResistant strains were renumbered.
  4. bResistant mutants selected from ILE-containing plates.
  5. cResistant mutants selected from ILF-containing plates.
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