Fig. 3: RhoA·GTP binds to the PLCε EF hands.
From: RhoA allosterically activates phospholipase Cε via its EF hands
a The 3.3 Å cryo-EM map of the RhoA·GTP–PLCε PH-C complex and b fitted with the ribbon diagram of the RhoA·GTP–PLCε PH-C complex. The domains in PLCε are colored as in Fig. 1a, and RhoA·GTP is shown in light blue. c Ribbon diagram of the RhoA·GTP–PLCε PH-C colored as in (a). The PLCε active site Ca2+ is shown as a black sphere, the E2α′ helix and adjacent loop that bind RhoA are shown in firebrick. Disordered regions, including the X–Y linker (hot pink) and Y-box (orange), are shown as dashed lines. RhoA·GTP is shown in light blue, Mg2+ as a black sphere, and GTP in light blue sticks. The N- and C-termini of each protein are labeled. d Density (gray mesh) for the E2α′ helix (shown in red) and e loop connecting the helix to the EF3/4 subdomain.
