Fig. 2: Periplasmic DypB3152 secretion is dependent on the VgrG proteins. | Communications Biology

Fig. 2: Periplasmic DypB3152 secretion is dependent on the VgrG proteins.

From: The secretion of Pseudomonas unconventional peroxidase facilitates extracellular carbon acquisition from heterogeneous lignin

Fig. 2

a, c, e Western blot analysis of the DypB3152 expression levels in the total cell lysates (Cell) and periplasmic (Peri) space of the relevant ∆vgrG mutants. The periplasmic protein, DsbA, was used as the periplasmic loading control. The cytoplasmic protein, RNA polymerase beta subunit (β-RNAP), was used as the loading control for total cell lysates. AR3152 was used as the control strain. Data are representative of three biological replicates. b, d, f The corresponding periplasmic DypB3152 activity of the ∆vgrG mutants. U/L: the DypB3152 activity per 1 L culture broth. “*”: p value < 0.05, “**”: p value < 0.01, “***”: p value < 0.001 by two-sided Student’s t-test. Data are presented as mean values ± standard deviation. n = 3 biological replicates.

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