Fig. 1: Effect of zingerone on RAW264.7 cell proliferation and bone mass in OVX mice. | Communications Biology

Fig. 1: Effect of zingerone on RAW264.7 cell proliferation and bone mass in OVX mice.

From: Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression

Fig. 1

A CCK-8 assay assessing the effect of different concentrations of zingerone on RAW264.7 cell proliferation (n = 3 independent biological replicates). B H&E-stained images of sections of major organs from mice (n = 6 per group). The data are presented as the mean ± standard deviation. Scale bar: 50 μm. C Sections of femoral bone tissue scanned by micro-CT (sagittal and cross-sectional views). Scale bar: 2 mm. D Micro-CT parameters of the femur showing BMD, Tb.Th, BV/TV, and Tb.Sp in the three groups (n = 6 per group). E Histological analysis of femoral tissue sections stained with H&E, Goldner’s trichrome, and TRAP for tissue pathology analysis. Scale bar: 1000 μm. Scale bar: 200 μm. F Immunofluorescence staining of OCN in bone tissue from the NC group, OVX group, and zingerone (Zin) group. Scale bar: 1000 μm. Scale bar: 50 μm. G Western blot analysis verifying the effects of zingerone on the expression of MMP9, RUNX2, ALP, BMP, and CTSK in bone tissue (n = 3). H Quantification of WB in (G). The values are presented as the mean ± standard deviation. *p < 0.05, **p < 0.01 and ***p < 0.001 compared to the model group; #p < 0.05, ##p < 0.01 and ###p < 0.001 compared to the control group.

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