Fig. 1: KAT8-mediated H3K27ac and H4K16ac are upregulated in WSSV-infected shrimp. | Communications Biology

Fig. 1: KAT8-mediated H3K27ac and H4K16ac are upregulated in WSSV-infected shrimp.

From: Histone H3K27 acetylation mediated by KAT8 maintains antiviral trained immunity in shrimp induced by inactivated white spot syndrome virus

Fig. 1

a Epigenetic modification patterns of pan-Hac, H3K27ac, H4K16ac, and H3K9ac in hemocytes of shrimp challenged with WSSV were analyzed by western blotting. a’ Statistical analysis of panel a based on three replicates. b–d RNA interference efficiency against Kat8 (b), Tip60 (c), and Kat2a (d) in shrimp hemocytes determined by qPCR. The qPCR data were normalized using Ef-1α as the endogenous control gene. e Abundances of H3K27ac and H3K9ac marks were measured in the hemocytes of shrimp after Kat8, Tip60, and Kat2a knockdown following WSSV infection via western blotting. dsGfp was used as the control. Bottom panel: Statistical analysis of the data in panel e based on three replicates. f Kat8-RNAi efficiency in hemocytes and intestinal tissues analyzed using qPCR; dsGfp injection was used as the control. g Modification levels of H3K27ac and H4K16ac were measured in the hemocytes and intestinal tissues of shrimp after Kat8 knockdown following WSSV infection, as determined by western blotting. dsGfp was used as a control. The samples were extracted at 24 hpi from Kat8 knockdown shrimp following WSSV infection. g’ The bands of three replicates of (g) were digitalized using ImageJ software and analyzed. The data are presented as the mean value ± SD (n = 3). P ≤ 0.05 (Student’s t-test).

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