Fig. 4: The glycolysis is enhanced in UV-WSSV-trained shrimp.

a, b Contents of glucose (a) and LA (b) in hemocytes of UV-WSSV trained shrimp collected at 3 h, 6 h, 12 h and 24 h after training. c Expression of Hk2, Pk, Ldh and Pdh at the mRNA level was measured in hemocytes of shrimp at 12 h after UV-WSSV training via qPCR with Ef-1α as the endogenous control gene. d Contents of PA in hemocytes collected at 6 h and 12 h after UV-WSSV training and at 3 h and 6 h after WSSV infection in UV-WSSV-trained shrimp. e Expression of Pk, Ldh and Pdh at the mRNA level was measured via qPCR in hemocytes of 2-DG-pretreated shrimp followed by UV-WSSV training, with Ef-1α as the endogenous control gene. f–h Contents of PA (f), LA (g) and acetyl-coenzyme A (Ac-CoA) (h) in hemocytes of 2-DG-pretreated shrimp followed by UV-WSSV training. i Influence of 2-DG on H3K27ac in shrimp. UV-WSSV-trained shrimp were pretreated with 2-DG. PBS was used as a control. At 12 h after training, the enrichment of H3K27ac in hemocytes of the shrimp was examined using western blotting. Bottom panel: Statistical analysis of the data in panel i based on three replicates. j Hif-1α and Hk2 expression was detected in Hif-1α-knockdown shrimp 12 h after UV-WSSV training via qPCR. k The abundance of H3K27ac was detected in Hif-1α-knockdown shrimp 12 h after UV-WSSV training using western blotting. Bottom panel: Statistical analysis of the data in panel k based on three replicates. The data are presented as the mean ± SD (n = 3) and significant differences were analyzed by Student’s t test.