Fig. 4: Identification of the hypothetical protein TgZct4.

TgZct4 is a transcription factor involved in the response to oxidative stress. A The TgZct4 amino acid sequence contains a typical Zn2-Cys6 Zinc cluster domain and a fungal-specific transcription factor domain, as revealed by Pfam. B Location of TgZct4 protein in cells. TgZct4-eGFP fusion protein was localized to the nucleus. C Transcriptional activation activity of the TgZct4 protein. The Y2H-BD transformant was used as a negative control. The transformants were streaked on YPDA, SD/Trp-/Agar and SD/Trp-/ X-α-Gal/Agar medium and incubated at 30 °C for 3 days in the darkness.