Fig. 7: Influence of Tgcat1 and Tgcat2 on the ability to tolerate H₂O₂.

A The TgCat1 amino acid sequence contains a “catalase domain” and a “catalase-related immune-responsive domain”, as revealed by Pfam. B The TgCat2 amino acid sequence contains a “catalase domain” and a “catalase-related immune-responsive domain”, as revealed by Pfam. C Hypersensitivity of the Tg WT, ΔTgcat1-1, ΔTgcat1-2, ΔTgcat2-1, ΔTgcat2-2, ΔTgcat1ΔTgcat2-1, and ΔTgcat1ΔTgcat2-2 strains cultivated on GSM plates supplemented with 0, 10, 20, and 40 mM H₂O₂ at 25 °C for 42 h in the darkness. D The relative inhibition rates of mycelial growth of Tg WT, ΔTgcat1-1, ΔTgcat1-2, ΔTgcat2-1, ΔTgcat2-2, ΔTgcat1ΔTgcat2-1, and ΔTgcat1ΔTgcat2-2 in the presence of 0, 10, 20, and 40 mM H₂O₂ cultivated for 42 h (n = 6 biologically independent samples). E Growth of Tg WT, ΔTgcat1-1, ΔTgcat1-2, ΔTgcat2-1, ΔTgcat2-2, ΔTgcat1ΔTgcat2-1, and ΔTgcat1ΔTgcat2-2 strains spores in GSM liquid medium supplemented with 0, 2.5, 5, 10, 15, and 20 mM H₂O₂ at 25 °C for 144 h in the darkness. F Growth curve of Tg WT, ΔTgcat1-1, ΔTgcat1-2, ΔTgcat2-1, ΔTgcat2-2, ΔTgcat1ΔTgcat2-1, and ΔTgcat1ΔTgcat2-2 strains spores in the presence of 0, 2.5, 5, 10, 15, and 20 mM H₂O₂ cultivated for 168 h and measured every 12 h (n = 7). ΔTgcat1-1 and ΔTgcat1-2, the single deletion mutants of gene Tgcat1; ΔTgcat2-1 and ΔTgcat2-2, the single deletion mutants of gene Tgcat2; ΔTgcat1ΔTgcat2-1 and ΔTgcat1ΔTgcat2-2, the double deletion mutants of both genes Tgcat1 and Tgcat2. The results are shown as the average values from biological duplications and the 1.96 * Standard Error for the Sample Mean. Different lowercase letters (a, b, c, d) indicate statistically significant differences at P < 0.05 in the growth of selected strains under the same concentration, based on one-way ANOVA followed by Tukey’s HSD test. The letters were assigned in descending order of sample means. Strains sharing the same letter were not significantly different. Exact P-values were provided in Supplementary Data 6.