Fig. 7: Natural variation in Or43a is involved in the regulation of group activity in flies.
From: Chemosensation drives divergent social behavior in Drosophila
A The SA-T2-GAL4-Poly(A) cassette in an intron of Or43a drives RedStinger expression in antennae. Scale bar: 200 μm. B–D The Or43a insertion mutant (C) displays reduced nighttime group hyperactivity (D) compared to the wDahomey control (B). For 24 h actograms, activity is shown as an average per fly ± s.e.m. in 1 h bins. D Response to group-housing, expressed as a ratio of activity per fly in groups vs. solo-housed (average ± s.e.m.) for the 12 h light (white) and dark (gray) periods. N = 17 vials per condition. E Effect of Canton-S (reference) vs. Dahomey alleles on nighttime group hyperactivity in 135 lines from the DGRP collection. Base numbering begins at the transcription start site. F The linear regression of nighttime group hyperactivity of the DGRP lines, categorized based on the number of Dahomey Or43a SNPs present in each line. R2: Correlation coefficient; p = 0.03 indicates the rejection of the null hypothesis that the slope ≤ 0. G Or43a SNPs associated with olfactory behavioral variation (BV) exhibit greater differences between alleles in the average nighttime Group/Solo activity ratio than SNPs not associated with BV. Males used in all experiments. *p < 0.05; ***p < 0.001 (Mann-Whitney U test). w, Da: wDahomey background.
