Fig. 6: HIIT increases bone formation and inhibits bone resorption via Gpr81 in OVX mice.
From: Exercise increases bone mass by lactate/Gpr81 signaling pathway
A In Gpr81−/− OVX mice, the effect of HIIT on bone formation rate was reduced. Images representative of calcein double labelling of spinal trabecular bone from OVX mice. Scale bars, 10 μm. B Bone formation parameters in vertebral sections of WT and Gpr81−/− OVX mice (bottom). *p < 0.05, **p < 0.01, ***p < 0.001, n = 6. C The regulatory effects of HIIT on osteoblast differentiation and osteoid formation are decreased in Gpr81−/− OVX mice. Goldner’s staining of mouse vertebral trabecular bone. Scale bars, 50 μm. D Histomorphometry parameters of vertebral bone. Ob.S/BS osteoblast surface per bone surface, OS/BS osteoid per bone surface. *p < 0.05, **p < 0.01, ***p < 0.001, n = 6. E The inhibitory effects of HIIT on osteoclast activity are decreased in Gpr81−/− mice. Trap staining representative images of mouse femoral trabecular bone. Scale bars, 200 μm. F Femur bone histomorphometry parameters of Trap staining. *p < 0.05, **p < 0.01, ***p < 0.001, n = 6. G Representative image of TRAP staining of the calvarias of OVX mice. Scale bars = 1 mm. H Percentage of TRAP-positive areas in the calvarias of the samples shown in (G). The data are presented as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, n = 6.
