Fig. 4: Plot of monocyte subclusters and Ifi27l2a gene expression. | Communications Biology

Fig. 4: Plot of monocyte subclusters and Ifi27l2a gene expression.

From: Up-regulation of Ifi27l2a expression in bone marrow monocytes contributes to glucocorticoid-induced bone loss

Fig. 4

A UMAP dimensional reduction plot of monocyte subclusters (with cluster annotation data). B Heat map of the marker genes (differentially expressed genes) for each monocyte cluster. The red box indicates the Ifi27l2a gene. C Violin plot of Ifi27l2a expression in the various monocyte subclusters. D Bar graph comparing the counts for each monocyte cluster in the GIO and control groups. E) UMAP maps comparing the expression of Ifi27l2a with that of the classical monocyte marker gene Lyz2. F UMAP maps comparing the expression of Ifi27l2a with the osteoclast differentiation marker genes Csf1r, Cxcr1, and Tnfrs11a. G Box plot comparing the proportion of cells in the GIO and control groups that are positive for the osteoclast differentiation marker genes Csf1r, Cxcr1, and Tnfrs11a. H, I Comparison of Ifi27l2a immunohistochemistry results in femoral bone marrow samples from normal and GIO mice and the corresponding statistical analysis. J, K Comparison of the immunofluorescence staining results for Ifi27l2a and Ly6c in femoral bone marrow samples from normal and GIO mice and the corresponding statistical analysis. Data were shown as the mean ± SD (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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