Fig. 1: Transcriptomic analysis of subcutaneous adipose tissue-derived mesenchymal stromal/stem cells (ASCsub).

Total RNAs were extracted from each ASC sample of individual donors with a body mass index (BMI) below 25 (ASCsub25, n = 5), equal or above 35 (ASCsub35, n = 5), and equal or above 40 (ASCsub40, n = 5) for transcriptomic analysis. A Venn diagram of co-expressed genes. B Volcano plots of significantly differentially expressed genes of ASCsub35 vs. ASCsub25 (left plot) and ASCsub40 vs. ASCsub25 (right plot). Upregulated genes are depicted in red, downregulated genes in green, and non‑changed genes in blue. p < 0.05. Significantly enriched “Gene Ontology” (GO) pathways are presented for ASCsub35 compared to ASCsub25 (C) and for ASCsub40 compared to ASCsub25 (D), sub-clustered into three ontological domains: molecular function (MF), cellular component (CC), and biological process (BP). The adjusted p value (padj) is displayed by a color code. Significantly enriched “Kyoto Encyclopedia of Genes and Genomes” (KEGG) pathways (except for PI3K-Akt ASCsub40 vs. ASCsub25) are presented for ASCsub35 compared to ASCsub25 (E) and for ASCsub40 compared to ASCsub25 (F). The p value is indicated by a color code. Abbreviations: neg. negative, PI3K-Akt phosphoinositide 3-kinase - protein kinase B, TGFβ transforming growth factor-β.