Fig. 3: Copper tolerance of copper homeostasis mutants under aerobic conditions.
From: A critical role of copper homeostasis in the virulence of Klebsiella pneumoniae
Copper tolerance of mutant strains was evaluated by spotting tenfold serial dilutions of logarithmic-phase cultures onto LB agar plates supplemented with copper (1–4 mM). The bacterial growth states of (a) Cue system, b Cus system, c Sod system, d ST258 and ST258 mutants, and (e) complementation strains are shown on plates with different copper concentrations. Bacterial counts from copper killing assays performed in liquid medium are shown for (f) Cue system, g Cus system, h Sod system, i ST258 and ST258 mutants, and (j) complementation strains. n = 3 biological replicates. Data are presented as mean ± SEM.
