Fig. 3: PDZK1 aggravates VSMC calcification by activating β-catenin. | Communications Biology

Fig. 3: PDZK1 aggravates VSMC calcification by activating β-catenin.

From: PDZ domain containing protein 1 aggravates mouse vascular smooth muscle cells calcification via PDZ1 domain mediated β-catenin stabilization and nuclear translocation

Fig. 3

a–f Representative western blot analysis and quantification of β-catenin and its phosphorylated protein (p-β-cateninSer552 and p-β-cateninS33/S37/T41), n = 3. Data are presented as relative fold change to Control: a MOVAS cell treated with or without Pi (2.6 mM) for 3, 5, or 7 days. b aortas from Pdzk1−/− mice vs WT mice. c, d The expression of total and nuclear β-catenin and its phosphorylated protein of sh-PDZK1 vs NC. e, f the expression of total and nuclear β-catenin and its phosphorylated protein of PDZK1-OE vs vector. g, h MOVAS transfected with shRNA of PDZK1 and treated with β-catenin recombinant protein combined with or without Pi (2.6 mM) for 7 days. g Representative Western blot analysis and quantification of PDZK1 and osteogenic markers (RUNX2, BMP2, and MSX2). n = 3. h ALP straining. n = 3. i, j MOVAS transfected with PDZK1 overexpression lentivirus and treated with E7386 (an inhibitor of β-catenin) combined with or without Pi (2.6 mM) for 7 days. i Representative western blot analysis and quantification of PDZK1 and osteogenic markers (RUNX2, BMP2, and MSX2). n = 3. j ALP straining. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, ns, not significant. The error bar refers to SD.

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