Fig. 1: Study design and RhCMV DNA loads.

A Twelve RhCMV-seronegative, immunocompetent rhesus macaque dams were intravenously inoculated with two RhCMV strains during early second trimester gestation. Blood, amniotic fluid (AF), urine, and saliva were collected weekly until elective C-section. Fetal and placental tissues were harvested at C-section. These samples were used in downstream analyses. Created in BioRender. Manuel, T. (2025) (https://BioRender.com/la7dz86). B RhCMV DNA copy numbers in plasma, saliva, and urine in individual animals. Mean of three to six technical PCR replicates for each data point shown. Viral loads expressed as mean copies of input RhCMV DNA per mL of plasma, and per µg of input DNA in saliva or urine.