Fig. 6: Viral load, histopathology, and immunochemistry analysis of GSHs after passive transfer of human IgG or IgA antibodies and SARS-CoV-2 challenge.
A Viral load was analyzed in oropharyngeal swab, nasal turbinate, and lungs (left to right). Levels of SARS-CoV-2 gRNA are expressed as log10 viral RNA copies/ml. Differences among groups were analyzed using Kruskal Wallis and Conover’s post-hoc tests corrected for FDR. Mean with standard deviation is shown. B Infectious virus titers were determined in the same tissues as in (A) at days 4, 7, and 14 post-infection. Data is shown as log10 median tissue culture infectious dose per milliliter (TCID50/ml). Dotted line indicates limit of quantification. Differences between groups were analyzed with the Kruskal–Wallis test followed by Conover’s post-hoc analysis adjusted by FDR. C Hematoxylin and eosin staining was used to perform a histopathologic analysis of nasal turbinate and lungs (left to right). Lesion score: 0 indicates absence of damage, while 1, 2, and 3 indicate mild, moderate, and severe lesions, respectively. D Nucleocapsid detection in nasal turbinate and lungs (left to right) by immunohistochemistry. Staining score: 0 indicates the absence of Nucleocapsid, while 1, 2, and 3 indicate low, moderate, and high levels of this viral antigen, respectively. Histopathologic and immunohistochemical differences among groups were analyzed using asymptotic generalized Pearson chi-squared test corrected for FDR. Mean with standard deviation is shown.
