Fig. 3: JNJ-9676 targets SARS-CoV M protein.
From: Structural insights into MERS and SARS coronavirus membrane proteins
a Chemical structure of JNJ-967619. b nanoDSF melting temperatures (Tm) of M protein dimethyl sulfoxide (DMSO) versus JNJ-9676; table indicates average Tm, ΔTm and standard deviations from three technical replicates. Mean differences between groups are calculated using paired t-test Holm-Sidak method where ** P ≤ 0.01, * P ≤ 0.05, ns P > 0.05. c Superimposed structural comparison of JNJ-9676 (orange) bound SARS-CoV-2 M protein (gray, PDB:8W2E) versus JNJ-9676 (magenta) bound SARS-CoV M protein (cyan, PDB: 9NZ3) in cartoon ribbon representation, highlighting JNJ-9676 binding pocket residues as sticks and lines. Key residue differences are highlighted with (*), and interactions are indicated with dashed lines (H-bonding: yellow; π-π stacking: green).
