Fig. 4: MIR22HG inhibits ENCC migration and proliferation by downregulating MPP3 expression. | Communications Biology

Fig. 4: MIR22HG inhibits ENCC migration and proliferation by downregulating MPP3 expression.

From: Extracellular vesicle-mediated transfer of MIR22HG inhibits the colonization of enteric neural crest cells in the colon by decreasing MPP3 expression

Fig. 4

A, B Overexpression of MIR22HG decreased the mRNA (n = 6) and protein (n = 3) levels of MPP3 in ENCCs. C, D MPP3 mRNA (n = 21) and protein (n = 3) levels were lower in HSCR-S segments than in control and HSCR-D segments. Introduction of MPP3 partially reversed the inhibitory effects of MIR22HG on ENCC migration (n = 5, scale bar=100 μm) (E), proliferation (n = 5, scale bar=50 μm) (F), and cytoskeleton formation (n = 3, scale bar=50 μm) (G). Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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