Fig. 4: DON inhibits glycolysis and histone lactylation in granulosa cells. | Communications Biology

Fig. 4: DON inhibits glycolysis and histone lactylation in granulosa cells.

From: Deoxynivalenol induces ferroptosis via inhibiting glycolysis-H3K18la-STEAP3 axis to promote ovary damage in piglets

Fig. 4: DON inhibits glycolysis and histone lactylation in granulosa cells.The alternative text for this image may have been generated using AI.

A Gene set enrichment analysis (GSEA) showing the top 10 functional enrichment results of all genes in the transcriptome. B Heatmap showing the relative levels of glycolysis-related metabolites before and after DON treatment. C, D Violin plots showing the relative levels of glucose and lactate in the two groups(n = 3). E ELISA measurement of lactate levels in ovarian GCs of different mouse groups (n = 9). Schematic diagram illustrating the mechanism of histone lactylation. F Schematic diagram of the structure and enzyme catalysis of histone lactylation, G Immunoblot analysis of pan-Kla in granulosa cells before and after DON treatment. H, I Prediction of potential lysine lactylation sites in porcine histone H3 and H4 using the CPLM database (http://cplm.biocuckoo.cn/index.php). J–L Western blotting analysis of site-specific histone lactylation in granulosa cells of control (CK) and DON-treated groups (n = 3). Bars indicate the mean ± SD. Statistical significance was analyzed via unpaired Student’s t-test, with significance levels denoted as follows: ns, p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (F) was created with FigDraw with copyright.

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