Fig. 8: STEAP3 deficiency disrupts iron homeostasis and induces ferroptosis in granulosa cells. | Communications Biology

Fig. 8: STEAP3 deficiency disrupts iron homeostasis and induces ferroptosis in granulosa cells.

From: Deoxynivalenol induces ferroptosis via inhibiting glycolysis-H3K18la-STEAP3 axis to promote ovary damage in piglets

Fig. 8: STEAP3 deficiency disrupts iron homeostasis and induces ferroptosis in granulosa cells.The alternative text for this image may have been generated using AI.

A WB analysis of STEAP3 protein levels in granulosa cells transfected with Si-NC or Si-STEAP3. B CCK8 assay showing cell viability in granulosa cells transfected with Si-NC or Si-STEAP3 (n = 6). C Immunofluorescence analysis of GPX4 protein expression in granulosa cells transfected with Si-NC or Si-STEAP3. (Scale bar:40 µm). D, E Representative images of Fe²⁺ and ROS levels and their relative fluorescence intensity in Si-NC and Si-STEAP3 granulosa cells observed by laser confocal microscopy (n = 3). Scale bars: 40 µm. F–H Representative images of lipid peroxidation and mitochondrial membrane potential levels and their relative fluorescence intensity in Si-NC, Si-STEAP3, and Si-STEAP3 + Fer-1 granulosa cells observed by laser confocal microscopy (n = 3). Scale bars: 40 µm. I–K Confocal microscopy images and their relative fluorescence intensity showing changes in lipid peroxidation and mitochondrial membrane potential in granulosa cells treated with OE-NC, OE-NC + DON, and OE-STEAP3 + DON. (Scale bar:40 µm) Bars indicate the mean ± SD. Statistical significance was analyzed via Student’s t-tests and one-way ANOVA with Tukey’s post hoc test. (ns, p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).

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