Fig. 2: Microbial taxonomic diversity (phylogenetic diversity) and indicated species of bacterial, fungal, and protistan communities in response to exposure to different plastic types (CK, PE, and PBAT).
a Phylogenetic diversity of bacterial, fungal, and protistan communities in response to plastic types exposure (n = 56 for bacteria and fungi; n = 52 for protists. CK treatment had no plastisphere samples). The top and bottom of the box represent the 75th and 25th quartiles, respectively, and the horizontal line within the box represents the median of each treatment. The asterisks indicate significant differences (****p < 0.0001), while “ns” represents non-significant differences (p > 0.05) among different treatments using the Wilcoxon rank-sum test. b Specific bacterial, fungal, and protistan species in response to different plastic types exposure using the linear discriminant analysis (LDA) effect size (LEfSe). Datasets were analyzed using the Kruskal–Wallis test (P < 0.05) to determine the differential specific species that were ranked according to the LDA score (log 10). c Cladogram illustrating phylogenetic relationships of specific species of bacterial, fungal, and protistan communities. Multilayer rings, from the inner to the outside layer, represent: (inner ring) phylum level taxonomy; (ring 1) plastic types categories based on LEfSe of specific ASVs, (ring 2–4) relative abundance of specific ASVs in response to different plastic types exposure.
