Fig. 2: Effects of temperature and the guide length on GgeAgo activity. | Communications Biology

Fig. 2: Effects of temperature and the guide length on GgeAgo activity.

From: A programmable pAgo nuclease with high activity and specificity for efficient DNA and RNA manipulation

Fig. 2

Effects of temperature on DNA (a) and RNA (b) cleavage by GgeAgo. c Effects of the 5’P-gDNA length on nucleic acid cleavage by GgeAgo. Target cleavage was performed at 50 °C with guides of various lengths as indicated. The assays in a–c were performed for 15 min (DNA Target) or 20 min (RNA Target) at indicated temperatures. Data are represented as the means ± SD from three independent experiments. d Co-purified nucleic acids from GgeAgo expressed in E. coli treated with enzymes as indicated. M, ssDNA marker; -, untreated; R, RNase A; D, DNase I; DR, both nucleases. The protein expression was induced for 16 h at 18 °C or 6 h at 37 °C.

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