Fig. 4: Effects of the 5’-nucleotide of the guide and mismatches in the guide-target duplex on GgeAgo activity. | Communications Biology

Fig. 4: Effects of the 5’-nucleotide of the guide and mismatches in the guide-target duplex on GgeAgo activity.

From: A programmable pAgo nuclease with high activity and specificity for efficient DNA and RNA manipulation

Fig. 4

Preferences for the 5’ nucleotide of the guide on DNA (a) and RNA (b) cleavage. The kobs values were determined from the single-exponential fits of the data. Effects of mismatches on DNA (c) and RNA (d) cleavage by GgeAgo. Data are the means ± SD from three independent measurements. The reactions were performed for 15 min (DNA Target) or 20 min (RNA Target) at 50 °C. *P < 0.05, ***P < 0.001 and ****P < 0.0001, compared to (c), the control reactions with guide containing no mismatches, using Student’s t test.

Back to article page