Fig. 5: Plasmid and linear dsDNA cleavage using GgeAgo. | Communications Biology

Fig. 5: Plasmid and linear dsDNA cleavage using GgeAgo.

From: A programmable pAgo nuclease with high activity and specificity for efficient DNA and RNA manipulation

Fig. 5: Plasmid and linear dsDNA cleavage using GgeAgo.The alternative text for this image may have been generated using AI.

a Schematic overview of the pUC19 target plasmid. The target sites are indicated by blue polylines, while percentages indicate the GC content of the 80-bp segments where target sites are located (Lower). The target sites (indicated in blue) and complementary DNA guides used in this experiment (indicated in red), and black triangles indicate the cleavage sites (Upper). b Plasmid cleavage at DNA sites with different GC content by GgeAgo-gDNA complexes at 70 °C for 30 min. Cleavage products were digested with Hind III or Sca I and analyzed by agarose gel electrophoresis. The product sizes are indicated below; positions of specific cleavage products are indicated with asterisks; M, molecular weight marker; Lin, linearized plasmid. c Linear dsDNA with different GC content cleavage by GgeAgo-gDNA complexes at 70 °C for 30 min.

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