Fig. 7: Virus RNA detection and variant alleles enrichment using GgeAgo.

a Schematic illustration of the virus RNA detection workflow using GgeAgo. The target RNA is pre-amplified by RT-PCR, followed by GgeAgo cleavage. The antisense strand of amplicon is cleaved by GgeAgo with a designed gDNA (black line), generating secondary gDNAs (gray background) that direct GgeAgo to cleave a fluorescent reporter. b Scheme illustration of virus RNA variant alleles enrichment using GgeAgo. Before RT-PCR amplification, the wild-type (WT) RNA is selectively removed by GgeAgo with a gDNA (Blue line) targeting WT RNA. The remaining steps are the same as in (a). c Detection sensitivity of the strategy in a using diluted synthetic mock SARS-Cov2 S gene WT RNA. d Detection of the D614G mutant RNA with different mutation frequencies by the strategy in (b). Data are the means ± SD from three biological replicates. *P < 0.05, **P < 0.01 and ****P < 0.0001, compared to the control, using Student’s t test.