Fig. 2: USP14 as a Potential DUB of ACTN1 and Its Role in Maintaining ACTN1 Stability.
From: USP14-mediated stabilization of ACTN1 maintains mesenchymal characteristics in glioblastoma
A Immunoprecipitation (IP) and IB analysis from HEK293T cells transfected with Flag-tagged DUBs (USP2, USP14, BAP1, ZRANB1) and probed with ACTN1 and Flag antibodies. B IB analysis of USP14 protein levels in NBTs and GBM subtypes, with β-actin control. C HEK293T cells were transfected with varying amounts of Flag-tagged USP14 (WT or C114A) for IB analysis. n = 3. D Quantitative analysis of USP14 and ACTN1 mRNA in LN229 and GBM2 cells with USP14 knockdown. n = 3. E IB showing ACTN1 protein reduction following USP14 knockdown in LN229 and GBM2 cells, with MG132 treatment reversing this effect. n = 3. F IB analysis of USP14 and ACTN1 in cells transfected with USP14-WT or C114A after USP14 knockdown. n = 3. G IB showing accelerated ACTN1 degradation following USP14 knockdown in LN229 and GBM2. n = 3. H Quantification of ACTN1 normalized to β-actin. I Overexpression of USP14-WT, but not USP14-C114A, stabilized ACTN1 in GBM1 cells, with quantification on the right. n = 3. **p < 0.01; ***p < 0.001; n.s., not significant.
