Fig. 8: IsoalloLCA promotes mitochondrial oxidative phosphorylation in macrophages. | Communications Biology

Fig. 8: IsoalloLCA promotes mitochondrial oxidative phosphorylation in macrophages.

From: Isoallolithocholic acid ameliorates intestinal inflammation via metabolically reprogrammed macrophages

Fig. 8: IsoalloLCA promotes mitochondrial oxidative phosphorylation in macrophages.The alternative text for this image may have been generated using AI.

Bone marrow-derived macrophages (BMDMs) were isolated from both wild-type (Wt) and Il10 knockout (Il10−/−) mice and induced for 9 days with GM-CSF. BMDMs were treated isoalloLCA (20 µM) with or without LPS (100 ng/mL) for 24 hours. a, b OCR data from a mitochondrial stress test and ECAR data from a glycolysis stress test of BMDMs. OCR oxygen consumption rate, ECAR extracellular acidification rate, FCCP phenylhydrazone, 2-DG 2-deoxyglucose, BASAL basal mitochondrial respiration, MRC maximal respiratory capacity, SRC mitochondrial ATP production, and spare respiratory capacity. c Western blot (WB) analysis for ETS2, HIF 1α and PFKFB3 in Wt and Il10−/− BMDMs. d Quantification of proteins levels in panel (c). The proteins levels were calculated against β-actin. Representative data shown in (a, b, and d) expressed as the mean ± standard error (SEM). Ordinary one-way ANOVA analysis for (d). ns, not significant (p ≥ 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

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