Fig. 5: Impact of ProQ mutations on mRNA binding.
From: Structural dependence of ProQ-mRNA interactions in live bacterial cells
A Effective diffusion coefficients (Deff) of WT and six mutant ProQ-PAmCherry proteins in the Δhfq background under NT and RIF treatment conditions. B Diffusion coefficients of the fast population (Dfast) (left) and the slow population (Dslow) (right) determined by the two-population CDF fitting for WT and six mutant ProQ-PAmCherry proteins in the Δhfq background under RIF treatment condition. C mRNA-associated fractions determined by the two-population CDF fitting for WT and six mutant ProQ-PAmCherry proteins in the Δhfq background under NT and RIF treatment conditions. D Number of spots per cell for WT and six mutant ProQ-PAmCherry proteins in the Δhfq background under NT condition. The number of spots were determined using 7500 frames consistently across all the samples. Experiments were performed for 4 to 9 biological replicates, with each data set containing ~1000 trajectories for Deff value calculation or ~2500 trajectories for mRNA-associated fraction calculation from ~50 cells. Error bars in all plots represent the s.d. from all biological replicates. P-values calculated using two-tailed t-test are labeled on top of each bar graph. All fitting results from both CDF fitting and saSPT analysis are reported in Supplementary Tables 1 and 2.
