Fig. 5: 124T sequence alters differentially expressed gene (DEG) profiles and reprogramming outcomes.

a, b Total number of DEGs in each cell type after transduction with Ascl1^SA6 (purple bars) or Ascl1^SA6-124T (blue bars) at day 7 (a) and day 50 (b). c, d Log2FC in gene expression, comparing iCre to Ascl1^SA6-iCre (c) or iCre to Ascl1^SA6-iCre-124T (d). Genes were categorized as associated with neurons, astrocytes or glycolysis. e, f Motor cortices transduced with mCh-124T, Ascl1^SA6-mCh, or Ascl1^SA6-mCh-124T were assayed for co-expression of mCherry with NPY (e), or NLGN1 (f). White arrows indicate marker⁺mCherry⁺; yellow arrows indicate marker-negative/mCherry⁺. Scale bars = 100 µm. The percentage of marker expression in mCherry⁺ cells was quantified (n = 3 animals each vector). Mean values and error bars representing the standard error of the mean (s.e.m.) were plotted. Statistical significance was assessed by a one-way ANOVA with Tukey’s post hoc multiple comparisons test.