Fig. 2: TERRA structure and sequence are crucial for driving multiphase formation.
From: Telomeric RNA and HP1α form interfacial clusters that stabilize HP1α-DNA condensates
a Confocal images of 20 µM HP1α (green) and 20 nM 4kbp DNA in the presence of 0.5 µM TERRA96 (magenta). Scale bar - 5 µm. b Confocal images of 20 µM HP1α-alexa488 (green) and 20 nM 4kbp DNA in the presence of 5 µM TERRA22 (magenta). Scale bar - 5 µm. c Confocal images of 20 µM HP1α-alexa488 (green) and 20 nM 4kbp DNA in the presence of 5 µM shuff-TERRA45 (magenta). Scale bar - 5 µm. d 1-H NMR spectra displaying G4 signature peaks in 10-12ppm region. e Confocal images of 20 µM HP1α-alexa488 (green) and 20 nM 4kbp DNA in the presence of 5 µM FMR1 RNA (magenta). Scale bar - 5 µm. f Confocal images of 20 µM HP1α-alexa488 (green) and 20 nM 4kbp DNA in the presence of 5 µM mut-TERRA45 (magenta). Scale bar - 5 µm. g Confocal images of 20 µM Mutant HP1α-3K-A (green) and 20 nM 4kbp DNA in the presence of 5 µM TERRA45 (magenta). Scale bar - 5 µm.
