Fig. 3: G4 structure of TERRA impacts the material properties of HP1α-RNA condensates.
From: Telomeric RNA and HP1α form interfacial clusters that stabilize HP1α-DNA condensates
a Brightfield images of condensates comprising 20 µM HP1α and TERRA45 across a range of RNA concentrations. Scale – 5 µm. b Brightfield images of condensates comprising 20 µM HP1α and mut-TERRA45 across a range of RNA concentrations. Scale – 5 µm. c Brightfield images showing that HP1α does not phase separate with control RNAs including homopolymers (polyA30, polyU30) and G-quadruplex RNAs (polyG15, Sc1, Map1b). Scale bar - 5 µm. d Time-lapse FRAP images showing HP1α recovery in TERRA45 condensates. Scale bar - 5 µm. e Time-lapse FRAP images showing HP1α recovery in mut-TERRA45 condensates. Scale bar - 5 µm. f FRAP recovery curves comparing HP1α dynamics in TERRA45 versus mut-TERRA45 condensates. Error bars represent SD (n = 3 independent experiments). g Time-lapse images showing differential salt sensitivity of 20 µM HP1α (magenta) condensate phases: TERRA45-rich phases persist while DNA-rich phases dissolve upon addition of 5 M sodium chloride. Scale bar - 10 µm.
