Table 3 Oligonucleotide sequences used in the study
Primer name | Sequence (5’-3’) |
|---|---|
CtpA-F | GGGAATTCCATATGTTCAGTCGTTTCTCTAATGT |
CtpA-R | CCCAAGCTTTTATTTCTTCTTAGGCGC |
CtpA K325A/Q329A-F | GGTGAAAAAACCTTTGGTGCGGGAAGCGTGGCGATGCTACTCCCTGTCAAT |
CtpA K325A/Q329A-R | ATTGACAGGGAGTAGCATCGCCACGCTTCCCGCACCAAAGGTTTTTTCACC |
CtpA H85A-F | TCTAATTTGGATGCGGCTTCAGCGTATTTGAAT |
CtpA H85A-R | ATTCAAATACGCTGAAGCCGCATCCAAATTAGA |
CtpA F105R-F | CAAACCGAGGGCGAAAGAGGGGGGCTTGGGATC |
CtpA F105R-R | GATCCCAAGCCCCCCTCTTTCGCCCTCGGTTTG |
CtpA R162A-F | GCGATCAATCTCATGGCCGGCAAGCCAAAGACC |
CtpA R162A-R | GGTCTTTGGCTTGCCGGCCATGAGATTGATCGC |
CtpA S300A-F | GTCAATGGCGGTTCAGCGGCCGCGAGCGAGATCGTCGCA |
CtpA S300A-R | TGCGACGATCTCGCTCGCGGCCGCTGAACCGCCATTGAC |
CtpA K325A-F | GGTGAAAAAACCTTTGGTGCGGGAAGCGTGCAGATGCTA |
CtpA K325A-R | TAGCATCTGCACGCTTCCCGCACCAAAGGTTTTTTCACC |
CtpA Q329A-F | TTTGGTAAGGGAAGCGTGGCGATGCTACTCCCTGTCAAT |
CtpA Q329A-R | ATTGACAGGGAGTAGCATCGCCACGCTTCCCTTACCAAA |
CtpA E382A-F | AAATTCAGCTTGAAAGCAGCGGATCTAAAACAC |
CtpA E382A-R | GTGTTTTAGATCCGCTGCTTTCAAGCTGAATTT |
CtpA E382R-F | AAATTCAGCTTGAAACGAGCGGATCTAAAACAC |
CtpA E382R-R | GTGTTTTAGATCCGCTCGTTTCAAGCTGAATTT |
