Fig. 4: Unraveling the molecular mechanism involved in TIL-lung cancer organoid interaction through single-cell RNA sequencing analysis.

A t-distributed Stochastic Neighbor Embedding (t-SNE) plot of all single cells analyzed, showing 28 distinct cell clusters. B A heatmap displaying the expression of canonical marker genes used to identify major cell lineages across the clusters. C The t-SNE plot from panel (A), with cells colored and annotated by their identified major cell type. D The t-SNE plot showing the integrated distribution of cells from different patient samples following batch effect correction. E The t-SNE plot with cells colored by experimental condition: before co-culture (Before-co), after co-culture (After-co), normal organoids (N_ORG), and tumor organoids (T_ORG). F The t-SNE plot with cells colored by the pathological subtype of the tumor of origin. G Violin plots comparing the expression of representative effector genes in T cells before versus after co-culture. H Bar chart showing the results of KEGG pathway enrichment analysis for genes differentially expressed in T cells after co-culture.