Fig. 6: Working model of the organoid-TIL co-culture platform. | Communications Biology

Fig. 6: Working model of the organoid-TIL co-culture platform.

From: Transcriptome profiling of tumor-infiltrating lymphocyte-mediated cytotoxicity against patient-derived lung cancer organoids

Fig. 6: Working model of the organoid-TIL co-culture platform.

This schematic summarizes the study’s workflow. Autologous TILs and tumor organoids are derived from NSCLC patients for co-culture. The resulting model is evaluated by in-vitro functional analyses (left; IHC, killing validation, flow cytometry) and scRNA-seq analyses (right; cell clustering, DEGs, pathway enrichment). These integrated analyses identify key genes, T cell population dynamics, and signaling pathways (center and bottom) that characterize the TIL-mediated anti-tumor response.

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