Fig. 3: Irisin attenuates the dexamethasone-induced reduction in myotube size. | Communications Biology

Fig. 3: Irisin attenuates the dexamethasone-induced reduction in myotube size.

From: Irisin regulates the phosphorylation of glucocorticoid receptor Ser212 and Ser234 and mediates glucocorticoid-induced muscle atrophy in mice

Fig. 3: Irisin attenuates the dexamethasone-induced reduction in myotube size.

A C2C12 cells were proliferated 3d to 100% and transferred to differentiation medium for 4 days, then treated with or without Dex and irisin for 24h, and cells were collected for WB, qRT-PCR and immunofluorescence. Created in BioRender. J, Y. (2025) https://BioRender.com/apfyv0h. B Immunofluorescence staining of eMyHC (green) and Hoechst (blue) in myotubes, Scale bar = 100 μM. C The frequency distribution of C2C12 myotubes diameter was analyzed. D The mean myotubes diameter was analyzed. E Western blot analyses of Atrogin-1, MuRF-1, p-AKT(Ser473), AKT and GAPDH in Dex and irisin-administered myotubes. F–H Protein levels were analyzed, n = 4 independent replicates. Data are presented as means ± SEM. Two-way ANOVA was used for statistical analysis.

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